RAPID PREPARATION OF A PANEL OF POLYCLONAL ANTIBODIES TO DROSOPHILA SEGMENTATION PROTEINS

We describe a method for rapidly raising a panel of high quality polyc lonal antibodies from bacterially expressed proteins. Approximately 1 2/3 days of preparation is required per protein. One step that speeds up the procedure is the visualization of purified bands by precipitate d sodium dodecyl sulfate (SDS). Antigenicity of the purified recombina nt proteins may be increased by precipitation in double-distilled wate r. The results of using the serums obtained for fluorescent staining o f Drosophila embryos are shown.