COLOCALIZATION OF CALRETININ AND PARVALBUMIN WITH NICOTINAMIDE ADENINE-DINUCLEOTIDE PHOSPHATE-DIAPHORASE IN TENCH MAUTHNER CELLS

The cc-localization of calretinin (CR) and parvalbumin (PV) immunoreac tivity with nicotinamide adenine dinucleotide phosphate-diaphorase (ND ) activity was analyzed in the Mauthner cells of the tench. Mauthner c ells were ND active, and ND staining was observed in the soma, axon ca p region, and axon of these neurons. CR cc-localized with ND in the ax on of the Mauthner cells but not in the cell body or in the dendrites, whereas PV immunoreactivity co-localized with ND in the soma, axon an d dendrites. The presence of two different calcium-binding proteins in the Mauthner cells indicates that these neurons need complex calcium- buffering systems. The co-localization of these calcium-binding protei ns with ND might suggests their involvement in nitric oxide-related ev ents. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.