SEIZURE ACTIVITY INDUCES PIM-1 EXPRESSION IN BRAIN

We recently identified KID-1, a previously undescribed protein kinase induced by depolarization in PC12 cells and brain (Feldman ct al,, 199 8), KID-1 shares a high degree of sequence homology with RIM-I, a prot o-oncogene previously reported to be expressed in hematopoietic and ge rm cells, We examined PIM-I expression in stimulated PC12 cells, brain s of kainic acid-treated rats, and a number of tissues from untreated rats, We now report that forskolin, but not depolarization or growth f actors, induces PIM-I expression in PC12 cells, PIM-1 is an immediate early gene induced in response to forskolin stimulation. We detect PIM -I mRNA in a number of unstimulated tissues and at low levels in unsti mulated brain, Systemic kainic acid administration to adult rats induc es PIM-I expression in the dentate gyrus region of the hippocampus. (C ) 1998 Wiley-Liss, Inc.