The flow cytometric determination of haemopoietic cells defined as CD3
4-expressing cells has greatly added to the improvement of the managem
ent of harvesting circulating haemopoietic cells for subsequent autolo
gous reinfusion in the setting of high-dose chemo/(radio)-therapy. Add
itionally, this flow cytometric determination has replaced, in some in
stitutions, the in-vitro culture test for CFU-GM as the measure to est
imate the haemopoietic potential of the cells to be reinfused/transpla
nted.