SCREENING OF A FOSMID LIBRARY OF MARINE ENVIRONMENTAL GENOMIC DNA FRAGMENTS REVEALS 4 CLONES RELATED TO MEMBERS OF THE ORDER PLANCTOMYCETALES

A fosmid library with inserts containing approximately 40 kb of marine bacterial DNA (J, L, Stein, T, L, Marsh, K, Y, Wu, H, Shizuya, and E. F. DeLong, J, Bacteriol. 178:591-599, 1996) yielded four clones with 16S rRNA genes from the order Planctomycetales. Three of the clones be long to the Pirellula group and one clone belongs to the Planctomyces group, based on phylogenetic and signature nucleotide analyses of full -length 16S rRNA genes. Sequence analysis of the ends of the genes rev ealed a consistent mismatch in a widely used bacterium-specific 16S rR NA PCR amplification priming site (27F), which has also been reported in some thermophiles and spirochetes.