DIFFERENTIATION OF DEXTRAN-PRODUCING LEUCONOSTOC STRAINS BY A MODIFIED RANDOMLY AMPLIFIED POLYMORPHIC DNA PROTOCOL

Authors
HOLT SM COTE GL
Citation
Sm. Holt et Gl. Cote, DIFFERENTIATION OF DEXTRAN-PRODUCING LEUCONOSTOC STRAINS BY A MODIFIED RANDOMLY AMPLIFIED POLYMORPHIC DNA PROTOCOL, Applied and environmental microbiology, 64(8), 1998, pp. 3096-3098
Citations number
21
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
64
Issue
8
Year of publication
1998
Pages
3096 - 3098
Database
ISI
SICI code
0099-2240(1998)64:8<3096:DODLSB>2.0.ZU;2-W
Abstract
Seven dextran-producing Leuconostoc strains were differentiated by usi ng a modified randomly amplified polymorphic DNA (RAPD) protocol that incorporated specific primers designed from conserved regions of dextr ansucrase genes. RAPD profiles showed intraspecies differences among t he Leuconostoc mesenteroides strains tested. This modified RAPD protoc ol will aid in the differentiation of polymer-producing leuconostocs, which are currently distinguished by time-consuming analyses of the de xtrans they synthesize.