COMPARISON OF C-18-CARBOXYPROPYLBETAINE AND GLASS BEAD DNA EXTRACTIONMETHODS FOR DETECTION OF MYCOBACTERIUM-BOVIS IN BOVINE-MILK SAMPLES AND ANALYSIS OF SAMPLES BY PCR

The purpose of this prospective study was to compare two different mil k preparation methods to assay for the presence of Mycobacterium bovis by PCR. Detection by a C-18-carboxypropylbetaine (CB-18)-based sample processing method was compared to extraction of DNA from milk with gl ass beads. Samples from 17 skin test-positive cattle were analyzed. Fo llowing CB-18 processing and glass bead extraction, the sensitivity of IS6110-based PCR was 94.1 and 58.8%, respectively (P < 0.025). Becaus e CB-18 processing will permit the proficient use of PCR for diagnosis and surveillance of bovine tuberculosis, it will contribute to the mo re efficient detection and control of tuberculosis.