We investigated the use of aerial thermal imagery to survey animals in
southwest Florida. Thermal signatures, in the 3- to 5-micron spectral
range, of deer (Odocoileus virginianus) were recorded on video tape d
uring flights along transects flown at 180-200 m altitude and at 74-93
km per hour. Our thermal imagery survey counted 42% more deer when co
mpared with standard visual aerial survey methods. Using thermal image
ry we successfully located radiocollared panthers (Puma concolor coryl
). Previous studies using imagery in the 8- to 14-micron spectral rang
e experienced difficulties differentiating objects of small thermal co
ntrast. We found the detectability of thermal contrast (Delta t) betwe
en biological objects and their background was sufficient to permit sp
ecies identification. These findings suggest that the use of 3- to 5-m
icron thermal imagery can be employed successfully to locate and count
animals.