THE DETECTION BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY OF P-30 AND 19-OH PROSTAGLANDIN F-1 F-2, IN THE PRESENCE OF A RANGE OF POSSIBLE CONTAMINANTS/

Authors
SUTTON JG BOSLEY C RANDS A
Citation
Jg. Sutton et al., THE DETECTION BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY OF P-30 AND 19-OH PROSTAGLANDIN F-1 F-2, IN THE PRESENCE OF A RANGE OF POSSIBLE CONTAMINANTS/, Science & justice, 38(3), 1998, pp. 157-164
Citations number
24
Categorie Soggetti
Pathology,"Medicine, Legal
Journal title
Science & justice → ACNP
ISSN journal
13550306
Volume
38
Issue
3
Year of publication
1998
Pages
157 - 164
Database
ISI
SICI code
1355-0306(1998)38:3<157:TDBEOP>2.0.ZU;2-Z
Abstract
The potential of specific human semen markers, P30 and 19-OH prostagla ndin F(1)alpha/F(2)alpha (PG F), ELISAs were compared in a blind trial . The PG F assay was shown to be at least ten times more sensitive tha n the P30 technique although cross reactivity problems with spermicida l creams and other possible domestic contaminants were identified. A s imple method for the removal of such contaminants using a phenyl boron ate column (PBA) was evaluated in an extensive blind trial.