D. Schontz et B. Rether, GENETIC-VARIABILITY IN FOXTAIL MILLET, SETARIA-ITALICA (L.) P. BEAUV.- RFLP USING A HETEROLOGOUS RDNA PROBE, Plant breeding, 117(3), 1998, pp. 231-234
Ribosomal DNA was surveyed in a world collection (43 lines) of Setaria
italica for variation in repeat unit length and restriction enzyme si
te variability using a non-radioactive wheat rDNA probe. Only two leng
ths of repeat units of about 7.9 or 7.6 kb were observed. The fact tha
t these two repeat unit lengths were not randomly dispersed throughout
the world collection suggests the existence of three genetic groups.
The central European lines and most western European accessions have o
nly the 7.6 kb repeat unit and most Asiatic lines have a 7.9 kb repeat
unit. Lines originating from the north or the south of Asia showed di
fferent numbers of BamHI fragments. The fact that the difference betwe
en the Asiatic and European pool is not continuous (7.9 or 7.6 kb) exc
ludes the hypothesis of domestication being based on the spread of an
initial population over Eurasia.