CURRENT RESPONSE AND DETERMINATION OF TRACES OF COENZYME-I AT A SILVER MICROELECTRODE

A useful current response of nicotinamide coenzyme I. at a silver micr oelectrode was obtained and, therefore, an electrochemical method has been developed for the determination of traces of the coenzyme by squa re wave adsorption stripping voltammetry. Under optimum conditions the proposed method provides a linear response over the coenzyme concentr ation in the range of 0.5-7.5 mu mol/L. The detection limit was 0.2 mu mol/L. The relative standard deviation was 3.5% for 6 successive dete rminations of the coenzyme at 1.0 mu mol/L. In view of the merits of t he electrochemical techniques and this microelectrode, this method wil l be of great usefulness in the biochemical and clinical fields in the future.