A. Wahl et al., POISONING WITH METHANOL AND ETHYLENE-GLYCOL - H-1-NMR SPECTROSCOPY ASAN EFFECTIVE CLINICAL TOOL FOR DIAGNOSIS AND QUANTIFICATION, Toxicology, 128(1), 1998, pp. 73-81
Analysis of serum and urine samples from three patients in a metabolic
acidotic state was performed using biochemical methods and proton nuc
lear magnetic resonance (H-1 NMR). Methanol and ethylene glycol were i
dentified by their singlet peaks in the H-1 NMR spectra recorded direc
tly from 0.5 mi of the collected samples. Quantification of these comp
ounds was obtained simultaneously with that of their metabolites, form
ate and glycolate respectively and of lactate and ethanol, the latter
being used as an antidote. The NMR results were found to be very simil
ar to the biochemical findings. The results presented here suggest tha
t H-1 NMR can be clinically useful since it quickly provides informati
on on the onset of metabolic acidosis and on the biotransformation of
xenobiotics. (C) 1998 Elsevier Science Ireland Ltd. All rights reserve
d.