GLUCOSE-MODIFIED LOW-DENSITY-LIPOPROTEIN ENHANCES HUMAN MONOCYTE CHEMOTAXIS

In diabetes mellitus the progression of atherosclerosis is accelerated . The interaction of glucose with atherogenic lipoproteins may be rele vant to the mechanisms responsible for this vascular damage. The aim o f this study was to examine the effect of glucose-modified low density lipoprotein (LDL) on human monocyte chemotaxis and to investigate the roles of oxidation and glycation in the generation of chemotactic LDL . Cu(II)-mediated LDL oxidation was potentiated by glucose in a dose-d ependent manner and increased its chemotactic activity. Incubation wit h glucose alone, under conditions where very little oxidation was obse rved, also increased the chemotactic property of LDL. Neither diethyle netriamine pentaacetic acid (DETAPAC) nor aminoguanidine, which both i nhibited LDL oxidation, completely inhibited the chemotactic activity of glycated oxidised LDL. The results suggest that both oxidation and glycation contribute to increased chemotactic activity.