SYNTHESIS OF A RADIOIODINATED PARK NUCLEOTIDE ANALOG - A NEW TOOL FORANTIBACTERIAL SCREEN DEVELOPMENT

Authors
EID CN NESLER MJ ZIAEBRAHIMI M WU CYE YAO R COX K RICHARDSON J
Citation
Cn. Eid et al., SYNTHESIS OF A RADIOIODINATED PARK NUCLEOTIDE ANALOG - A NEW TOOL FORANTIBACTERIAL SCREEN DEVELOPMENT, Journal of labelled compounds & radiopharmaceuticals, 41(8), 1998, pp. 705-716
Citations number
12
Categorie Soggetti
Chemistry Analytical","Chemistry Medicinal","Biochemical Research Methods","Pharmacology & Pharmacy
Journal title
Journal of labelled compounds & radiopharmaceuticals → ACNP
ISSN journal
03624803
Volume
41
Issue
8
Year of publication
1998
Pages
705 - 716
Database
ISI
SICI code
0362-4803(1998)41:8<705:SOARPN>2.0.ZU;2-C
Abstract
The Park nucleotide is an important biological building block used in the construction of bacterial cell walls. Herein, we describe the synt hesis of a radiolabeled Park nucleotide analog, yl-Ala-(D)-iso-Glu-Lys -(D)-Ala-(D)-Ala-OH-[I-125], using electrophilic destannylation. Anti- Park nucleotide antibody binding assays using a scintillation proximit y assay (SPA) system showed good recognition of the radiolabeled surro gate. This methodology could be used for establishing a screen to iden tify inhibitors of peptidoglycan biosynthesis.