1. The effect of in vitro glycation on delta-aminolevulinic dehydratas
e (ALA-D) under several experimental conditions was investigated. When
preincubated with 500 mM glucose at 37 degrees C for 20 hr, ALA-D was
80% inactivated and glycated hemoglobin levels were increased more th
an fourfold. 2. Thiobarbituric acid species were not modified during g
lycation; therefore ALA-D inactivation cannot be attributed to glucose
autoxidation. 3. Acetyl salicylic acid was effective in preventing bo
th hemoglobin glycation and ALA-D inactivation by glucose. 4. A method
has been developed for measuring protein glycation in vitro, in a cru
de preparation of red blood cells, which can also he applied to sugars
other than glucose. (C) 1998 Elsevier Science Inc.