N. Shintani et al., INVOLVEMENT OF CD4(-CELLS IN THE DEVELOPMENT OF DEXTRAN SULFATE SODIUM-INDUCED EXPERIMENTAL COLITIS AND SUPPRESSIVE EFFECT OF IGG ON THEIR ACTION() T), General pharmacology, 31(3), 1998, pp. 477-481
1. To clarify the role of T cells in the development of dextran sulfat
e sodium (DSS) induced colitis, T cells from colitis mice were primed
with DSS pulsed macrophages in vitro and then transferred into normal
mice. In addition, to determine whether the target cell of immunoglobu
lin G (IgG) is the T cell, the extent of T cell proliferation induced
by pulsed macrophages was examined after preincubation with IgG. 2. Wh
en mice receiving the primed T cells were treated with oral DSS, colit
is symptoms were more severe than in animals treated with oral DSS onl
y. This activity of primed T cells was reduced by depletion from the c
ells of CD4(+) but not CD8(+) cells. 3. The proliferation of T cells f
rom colitis mice induced by pulsed macrophages was inhibited by T cell
preincubation with homologous IgG. 4. The results suggest that CD4(+)
T cells play an important role in the development of DSS-induced expe
rimental colitis and that IgG may modulate the development of colitis
through interaction with pathogenic T cells. (C) 1998 Elsevier Science
Inc.