VALIDATION OF ANTIVASCULAR ENDOTHELIAL GROWTH-FACTOR (ANTI-VEGF) ANTIBODIES FOR IMMUNOHISTOCHEMICAL LOCALIZATION OF VEGF IN TISSUE-SECTIONS- EXPRESSION OF VEGF IN THE HUMAN ENDOMETRIUM
L. Zhang et al., VALIDATION OF ANTIVASCULAR ENDOTHELIAL GROWTH-FACTOR (ANTI-VEGF) ANTIBODIES FOR IMMUNOHISTOCHEMICAL LOCALIZATION OF VEGF IN TISSUE-SECTIONS- EXPRESSION OF VEGF IN THE HUMAN ENDOMETRIUM, Journal of pathology, 185(4), 1998, pp. 402-408
Transient transfection of COS-1 cells followed by fixation, embedding
in paraffin, and immunohistochemistry has identified anti-vascular end
othelial growth factor (anti-VEGF) mouse monoclonal antibodies that ef
ficiently immunostain VEGF in paraffin-embedded tissue sections, Immun
ohistochemical localization of VEGF in 34 specimens of normal human en
dometrium that had been collected at different stages of the menstrual
cycle was then performed. VEGF was present at all stages of the cycle
, but both the pattern and the intensity of staining varied. Thus, VEG
F expression occurred predominantly in the endometrial epithelium and
while weak in the proliferative phase, wits strong in the secretory ph
ase. VEGF expression in the stroma was weaker than in the proliferativ
e phase glands and did not change throughout the cycle, These findings
are in agreement with reports of VEGF mRNA expression in the endometr
ium, hot disagree with previous immunohistochemical studies that emplo
yed an immunohistochemically unvalidated antiserum, This study has sha
wm that the commercially available anti VEGF monoclonal antibody M293
is excellent for the immunohistochemical localization of VEGF in paraf
fin sections. (C) 1998 John Wiley & Sons, Ltd.