VALIDATION OF ANTIVASCULAR ENDOTHELIAL GROWTH-FACTOR (ANTI-VEGF) ANTIBODIES FOR IMMUNOHISTOCHEMICAL LOCALIZATION OF VEGF IN TISSUE-SECTIONS- EXPRESSION OF VEGF IN THE HUMAN ENDOMETRIUM

Citation
L. Zhang et al., VALIDATION OF ANTIVASCULAR ENDOTHELIAL GROWTH-FACTOR (ANTI-VEGF) ANTIBODIES FOR IMMUNOHISTOCHEMICAL LOCALIZATION OF VEGF IN TISSUE-SECTIONS- EXPRESSION OF VEGF IN THE HUMAN ENDOMETRIUM, Journal of pathology, 185(4), 1998, pp. 402-408
Citations number
9
Categorie Soggetti
Pathology
Journal title
ISSN journal
00223417
Volume
185
Issue
4
Year of publication
1998
Pages
402 - 408
Database
ISI
SICI code
0022-3417(1998)185:4<402:VOAEG(>2.0.ZU;2-Q
Abstract
Transient transfection of COS-1 cells followed by fixation, embedding in paraffin, and immunohistochemistry has identified anti-vascular end othelial growth factor (anti-VEGF) mouse monoclonal antibodies that ef ficiently immunostain VEGF in paraffin-embedded tissue sections, Immun ohistochemical localization of VEGF in 34 specimens of normal human en dometrium that had been collected at different stages of the menstrual cycle was then performed. VEGF was present at all stages of the cycle , but both the pattern and the intensity of staining varied. Thus, VEG F expression occurred predominantly in the endometrial epithelium and while weak in the proliferative phase, wits strong in the secretory ph ase. VEGF expression in the stroma was weaker than in the proliferativ e phase glands and did not change throughout the cycle, These findings are in agreement with reports of VEGF mRNA expression in the endometr ium, hot disagree with previous immunohistochemical studies that emplo yed an immunohistochemically unvalidated antiserum, This study has sha wm that the commercially available anti VEGF monoclonal antibody M293 is excellent for the immunohistochemical localization of VEGF in paraf fin sections. (C) 1998 John Wiley & Sons, Ltd.