SEROLOGICAL ASSOCIATION BETWEEN SPIRORCHIDIASIS, HERPESVIRUS-INFECTION, AND FIBROPAPILLOMATOSIS IN GREEN TURTLES FROM FLORIDA

Citation
Lh. Herbst et al., SEROLOGICAL ASSOCIATION BETWEEN SPIRORCHIDIASIS, HERPESVIRUS-INFECTION, AND FIBROPAPILLOMATOSIS IN GREEN TURTLES FROM FLORIDA, Journal of wildlife diseases, 34(3), 1998, pp. 496-507
Citations number
32
Categorie Soggetti
Veterinary Sciences
ISSN journal
00903558
Volume
34
Issue
3
Year of publication
1998
Pages
496 - 507
Database
ISI
SICI code
0090-3558(1998)34:3<496:SABSH>2.0.ZU;2-4
Abstract
Serodiagnostic tests for detecting green turtle (Chelonia mydas) antib ody responses were developed to test the strength of association betwe en exposure to spirorchid trematode antigens or herpesvirus antigens a nd having green turtle fibropapillomatosis (GTFP). Plasma samples from 46 captive-reared green turtles, including paired pre- and 1-yr post- inoculation samples from 12 turtles with experimentally induced GTFP. were found by enzyme-linked immunosorbent assay (ELISA) to be negative for antibodies to adult spirorchid (Learedius learedi) antigens. In c ontrast, all 12 turtles that developed experimentally induced GTFP con verted within 1 yr from having negative to positive antibody reactivit y to GTFP-associated herpesvirus antigens, whereas the three controls and four turtles that failed to develop tumors remained negative. Plas ma samples from 104 free-ranging green turtles from two Florida (USA) coastal feeding grounds with different GTFP prevalences were tested by ELISA for antibodies to L. learedi adult antigens: and there was no s tatistically significant association between antibody prevalence and s ampling site. When a low optical density cutoff value (0.15) was used to interpret ELISA results, 98% of the turtles from each site were spi rorchid antibody-positive and there was no association between antibod y reactivity to spirorchids and GTFP status. When a higher negative cu toff value was used, however, a statistically significant association between antibody reactivity to spirorchids and GTFP-free status was fo und. These results suggest that spirorchids do not have a role in GTFP pathogenesis. All 20 of the tumor-bearing lagoon turtles had antibodi es to herpesvirus antigens whereas only two (10%) of the turner-free r eef turtles had detectable anti-herpesvirus reactivity. The strong ass ociation between antibody reactivity to herpesvirus antigens and GTFP status in both captive-reared and free-ranging turtles is consistent w ith the hypothesis that the transmissible agent that causes GTFP is a herpesvirus.