THE CYP2 FAMILY - MODELS, MUTANTS AND INTERACTIONS

1. The construction of three-dimensional models of mammalian cytochrom es P450 from the CYP2 family is reported based on protein sequence ali gnment with CYP102, a bacterial P450 of known crystal structure. 2. Th e homology models of CYP2 family enzymes appear to show self-consisten cy with the currently accumulated information from site-directed mutag enesis and chemical modification of amino acid residues known to affec t redox partner interactions. 3. The generation of these models from t he recently reported crystal structure of substrate-bound CYP102 enabl es the exploration of likely active site contacts with specific substr ates of CYP2 family isozymes.