SEQUENCING AND PHYLOGENETIC ANALYSIS OF THE PROTEASE GENE, AND GENETIC-MAPPING OF BOVINE ADENOVIRUS TYPE-10 DEFINE ITS RELATEDNESS TO OTHERBOVINE ADENOVIRUSES

The complete genome of a bovine adenovirus (BAV) type 10 isolate was m olecularly cloned and partially sequenced. The encoded proteins were p redicted by computer analysis of the DNA sequences of the ends or the entire length of the cloned viral fragments, and thus a rough genetic map was constructed. The protease gene of BAV-10 was completely sequen ced and used in phylogenetic analysis. Based on the results of the phy logenetic analysis, and the location and presence of certain genes tho ught to be specifically characteristic of subgroup 1 or subgroup 2 BAV s, it could be concluded that, in spite of the striking similarity in certain biological properties, BAV-10 is not related to subgroup 2 BAV s as originally described. It does not however fit clearly into subgro up 1 either, the members of which show closer relationship with human adenoviruses. BAN-10 therefore should best be considered as the first member of a third subgroup of BAVs. (C) 1998 Elsevier Science B.V. All rights reserved.