CDNA CLONING OF A NEW LOW-DENSITY-LIPOPROTEIN RECEPTOR-RELATED PROTEIN AND MAPPING OF ITS GENE (LRP3) TO CHROMOSOME BANDS 19Q12-Q13.2

We report herein the cDNA cloning of a novel member of the low-density lipoprotein receptor (LDLR) family termed LDLR-related protein 3 (LRP 3). Human and rat LRP3 cDNAs encode a 770-amino-acid type 1 membrane p rotein with the following regions: a putative signal sequence, two iso leucine/leucine/valine-rich regions with an RGD sequence, two ligand-b inding repeat regions, a putative transmembrane region, and a proline- rich cytoplasmic region with a tyrosine-based internalization signal. Fluorescence in situ hybridization of human chromosomes revealed that the human LRP3 gene mapped to chromosome bands 19q12-q13.2. LRP3 trans cript was detected in a wide range of human tissues, with the highest expression in skeletal muscle and ovary. Despite the presence of the l igand-binding repeats, CHO cells transfected with LRP3 did not bind be ta-migrating very-low-density lipoprotein or receptor-associated prote in. (C) 1998 Academic Press.