Cytokines participate in the induction and effector phases of all immu
ne and inflammatory responses. They are therefore obvious tools and ta
rgets for strategies designed to promote, inhibit or redirect these re
sponses. However, the complexity of the cytokine network has hindered
the widespread clinical application of many cytokines and it has becom
e clear that a deeper understanding of the normal operation of this sy
stem in health and disease is needed for the therapeutic potential of
cytokines to be fully realized. This review summarizes some of the pri
nciples that are now thought to underlie the diverse functions of the
interleukins, interferons, colony-stimulating factors and tumour necro
sis factors in immune and inflammatory reactions in vivo. Genetic and
structural relationships between these cytokines, the regulation of th
eir synthesis, and the structures and functions of their receptors are
outlined. Current knowledge of these parameters suggests ways in whic
h. multiple positive and negative regulatory mechanisms are integrated
to balance cytokine benefits and harm under physiological conditions
and offers new prospects for rational exploitation of this system.