AN ALPHA-L-FUCOSIDASE FROM PENICILLIUM MULTICOLOR AS A CANDIDATE ENZYME FOR THE SYNTHESIS OF A (1-]3)-LINKED FUCOSYL OLIGOSACCHARIDES BY TRANSGLYCOSYLATION

A new alpha-L-fucosidase was partially purified from the culture broth of Penicillium multicolor, which was available commercially as a free ze dried powder by the name of Lactase-P(TM). This enzyme catalysed th e transglycosylation of fucose residue of p-nitrophenyl-alpha-L-fucopy ranoside to give alpha-L-Fuc-(1-->3)-D-Glc or alpha-L-Fuc-(1-->3)-D-Gl cNAc regioselectively. This enzyme was more stable in the organic co-s olvents than the alpha-fucosidase from Aspergillus niger, which was al so proposed previously by us as an enzyme to produce fucosyl oligosacc harides. (C) 1998 Elsevier Science Ltd. All rights reserved.