STUDIES ON THE PREPARATION AND IN-VITRO CYTOTOXICITY OF POLY(L-ASPARTIC ACID)DERIVATIVES-CIS-PLATIN CONJUGATES

Three alpha,beta-poly(L-aspartic acid) derivatives-cis-platin conjugat es were synthesized by conjugation of alpha,beta-poly(L-aspartic acid) , (alpha,beta,L-aspartic acid)-[alpha,beta,L-(N-hydroxyethyl)aspartami de] copolymer and spartamide]-[alpha,beta,L-(N-succinyl)aspartamide] c opolymer with cis-dichlorodiammine platinum( II) respectively. The pol ymer carriers were characterized by IR, GPC and intrinsic viscosity. T he influence of pH on the conjugation reaction was studied. Cytotoxici ty of polymer-cis-platin conjugates was tested against cervix uteri He la cells in vitro, The cytotoxicity of polymer-cis-platin conjugates i ncreases with the increase of concentration of the congugates, but low er than cis-platin of the corresponding concentration.