SEPARATION OF THE BRANCH CHAIN HEXOSE, HAMAMELOSE, BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, AND AMPEROMETRIC DETECTION OF HAMAMELOSE AND RELATED-COMPOUNDS

Citation
Pj. Andralojc et al., SEPARATION OF THE BRANCH CHAIN HEXOSE, HAMAMELOSE, BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, AND AMPEROMETRIC DETECTION OF HAMAMELOSE AND RELATED-COMPOUNDS, Journal of chromatography, 814(1-2), 1998, pp. 105-110
Citations number
12
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Volume
814
Issue
1-2
Year of publication
1998
Pages
105 - 110
Database
ISI
SICI code
Abstract
The identification and purification of the branch chain hexose hamamel ose (2-hydroxymethyl-D-ribose) from plant leaves is described. The pro cedure includes metabolite extraction in acid and a series of isocrati c HPLC separations. Amperometric detection of hamamelose is shown to b e quantitative, having a detection limit below 1 nmol. Similar detecti on of the related compound, 2-carboxy-D-arabinitol, and its monophosph ate ester, yields unusually small peaks. (C) 1998 Elsevier Science B.V . All rights reserved.