INHERITANCE AND LINKAGE RELATIONSHIPS OF ALLOZYMES, AND ESTIMATION OFOUTCROSSING RATES IN A SEED ORCHARD OF CUNNINGHAMIA-KONISHII HAY

Inheritance and linkage relationships of 11 polymorphic loci from 9 en zyme systems in Cunninghamia konishii were analyzed by horizontal star ch gel electrophoresis using megagametophyte haploid tissues collected from Chuyunshan seed orchard, Taiwan. The outcrossing rate was also e stimated based on multilocus and single-locus models. The observation of segregated female gametophytes of heterozygous trees revealed simpl e MENDELIAN inheritance for most of the allozyme loci. Linkage relatio nships were examined for 39 pairs of polymorphic allozyme loci. Three pairs with significant joint segregation were detected: Mdh-1/6Pgd-2 w ith a recombination value (R) 0.098, Fest-1/Fest-3 with R = 0.168, and Fest-2/Fest-3 with R = 0.038. Single-locus estimates (t(s)) of outcro ssing rate varied between 0.640 and 0.991 with an average of 0.847. Co mpared with the single-locus outcrossing rate estimates, the multilocu s estimate (t(m) = 0.902) indicated that a part of the inbreeding may be biparental.