ISOLATION OF AMINO-ACIDS FROM NATURAL SAMPLES USING SUBLIMATION

Authors
Citation
Dp. Glavin et Jl. Bada, ISOLATION OF AMINO-ACIDS FROM NATURAL SAMPLES USING SUBLIMATION, Analytical chemistry (Washington), 70(15), 1998, pp. 3119-3122
Citations number
14
Categorie Soggetti
Chemistry Analytical
ISSN journal
00032700
Volume
70
Issue
15
Year of publication
1998
Pages
3119 - 3122
Database
ISI
SICI code
0003-2700(1998)70:15<3119:IOAFNS>2.0.ZU;2-2
Abstract
Amino acids have appreciable vapor pressures above 150 degrees C and w ill sublime under partial vacuum at elevated temperatures without any racemization or decomposition. The recoveries of several amino acids i ncluding aspartic acid, serine, glycine, alanine, ol-aminoisobutyric a cid, and valine were optimized by varying the temperature and duration of sublimation. Sublimation has been shown to be a rapid and effectiv e technique for the isolation of amino acids from natural samples for enantiomeric analyses and a good substitute for conventional cation-ex change desalting techniques.