FREQUENT REARRANGEMENTS OF CHROMOSOME-1, CHROMOSOME-7, AND CHROMOSOME-8 IN PRIMARY LIVER-CANCER

Fifteen primary liver carcinomas (PLCs), including 12 hepatocellular c arcinomas and three cholangiocellular carcinomas, were investigated cy togenetically after short-term culture. Ten tumors displayed clonal ch romosomal abnormalities, whereas only normal karyotypes were detected in four cases, and one sample failed to grow in vitro. Structural rear rangements most often involved chromosomes 1, 7, and 8 and chromosome bands 1p36, 1q25, 3q10, 5q13, 6p10, 7p15, 7q22, 7q32, 8q10, 8q13, 14q1 0, and 17p11. Frequent genomic imbalances included gains of 1q, 3q, 6p , 7p, and 8q and losses of 1p, 8p, 10q, 14p, 17p, and 19p. A compilati on of findings for all 19 cytogenetically abnormal PLCs reported to da te, including the present cases, reveals that structural aberrations p articularly affect 1p11, 1p22, 1p32, 1p34, 1p36, 1q25, 7p15, 7q22, 8q1 0, 8q13, 14q10, 16q24, and 17p11, and that the abnormalities frequentl y result in overrepresentation of 1q, 3q, 6p, 7p10-14, 8q, and 17q and underrepresentation of 1p34-36, 6q27, 7q32-qter, 8p, 13p, 14p, 16q24, and 17p. These genomic regions are likely to harbor genes of importan ce in hepatocarcinogenesis, and the present cytogenetic mapping may he nce be of value for further molecular genetic investigations of PLC. ( C) 1998 Wiley-Liss, Inc.