THE EFFECT OF CYTOKINE MEDIATORS ON PROSTAGLANDIN INHIBITION BY HUMANDECIDUAL CELLS

OBJECTIVE: The purpose of this study was to determine the inhibitory e ffects of anti-interleukin-1 beta and transforming growth factor-beta( 2) on the production of interleukin-1 beta and prostaglandin E-2 by hu man decidual cells. STUDY DESIGN: Decidual membranes were obtained fro m 30 patients between 37 and 40 weeks' gestation. Decidual cells were isolated and grown in culture. Incubations were conducted with lipopol ysaccharide as a control, lipopolysaccharide plus anti-interleukin-1 b eta, and lipopolysaccharide plus transforming growth factor-beta(2), r espectively. Interleukin-1 beta and prostaglandin E-2 were measured by enzyme-linked immunosorbent assay and radioimmunoassay. RESULTS: At t he end of a 24-hour culture with lipopolysaccharide plus anti-interleu kin-1 beta, anti-interleukin-1 beta decreased the lipopolysaccharide-i nduced prostaglandin E-2 production significantly at the concentration s of 50 ng/mL, 100 ng/mL, and 500 ng/mL (P<.05) and also decreased the lipopolysaccharide-induced interleukin-1 beta production significantl y at the concentrations of 10 ng/mL, 50 ng/mL, and 100 ng/mL (P<.05). In the medium containing lipopolysaccharide plus transforming growth f actor-beta(2), transforming growth factor-beta(2) also decreased the l ipopolysaccharide-induced prostaglandin E-2 production significantly a t the concentrations of 1 ng/mL and 10 ng/mL (P<.05) but increased the lipopolysaccharide-induced interleukin-1 beta production significantl y at the concentration of 1 ng/mL (P<.05). CONCLUSIONS: Lipopolysaccha ride-induced prostaglandin E-2 production by human decidual cells in v itro may be prevented by anti-interleukin-1 beta and transforming grow th factor-beta(2), and interleukin-1 beta production may be prevented by anti-interleukin-1 beta.