ASSIGNMENT OF THE HUMAN SEROTONIN-4 RECEPTOR GENE (HTR4) TO THE LONG ARM OF CHROMOSOME-5 (5Q31-Q33)

In the present study, we report the chromosomal localization of the hu man 5-HT4 receptor gene (HTR4) by the analysis of somatic cell hybrids . Based upon genomic sequences of the HTR4 gene, a primer set was sele cted that reacted with human genomic DNA but not mouse or hamster geno mic DNA. Using monochromosomal hybrid cell lines of the NIGMS Mapping Panel 2 we localized the HTR4 gene to human chromosome 5. To confirm t he localization on chromosome 5 and to further sublocalize the HTR4 ge ne, the radiation hybrid panel RH3 was similarly analysed. Significant linkage was obtained to genetic marker D5S2654. This localizes the HT R4 gene to human chromosome 5q31 - q33.