COPURIFICATION OF P6, MRP8, AND MRP14 FROM HUMAN GRANULOCYTES AND SEPARATION OF INDIVIDUAL PROTEINS

Authors
VANDENBOS C RAMMES A VOGL T BOYNTON R ZAIA J SORG C ROTH J
Citation
C. Vandenbos et al., COPURIFICATION OF P6, MRP8, AND MRP14 FROM HUMAN GRANULOCYTES AND SEPARATION OF INDIVIDUAL PROTEINS, Protein expression and purification (Print), 13(3), 1998, pp. 313-318
Citations number
24
Categorie Soggetti
Biochemical Research Methods",Biology,"Biothechnology & Applied Migrobiology
Journal title
Protein expression and purification (Print) → ACNP
ISSN journal
10465928
Volume
13
Issue
3
Year of publication
1998
Pages
313 - 318
Database
ISI
SICI code
1046-5928(1998)13:3<313:COPMAM>2.0.ZU;2-7
Abstract
A method is described for purification of P6, MRP8, and MRP14, three c alcium-binding proteins assigned to the S100 protein family. The purif ication procedure included preparation of human granulocytes, ammonium sulfate precipitation, and anion-exchange chromatography and resulted in the copurification of P6, MRP8, and MRP14. Individual proteins wer e separated by either preparative isoelectric focusing or preparative SDS-PAGE. The procedure was carried out in the course of 4 days and yi elded several milligrams of essentially pure P6, MRP8, and MRP14 in ei ther native Or denatured form. (C) 1998 Academic Press.