RHIZOGENESIS IN BUCKWHEAT THIN-CELL-LAYER EXPLANTS - EFFECT OF PLANT OLIGOSACCHARIDES

Thin cell-layer explants from Fagopyrum esculentum Moench hypocotyls c ultured in liquid medium without growth regulators have been shown to be able to form roots. The intensity of this process depended upon the size of the explant and the length of the hypocotyl. Six diverse olig osaccharide fractions separated from the pea shoot tissues differently effected the rhizogenesis in this system. One fraction from the cell wall pectin and two from the cell sap stimulated this process. Another fraction from the cell wall pectin and two other from the cell sap in hibited explant rooting. One fraction from the cell sap differently in fluenced two separate stages of root development. These results indica te that the oligosaccharide fragments can be regulators of rhizogenesi s. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.