TETRAETHYLENE GLYCOL-DERIVED SPACER FOR OLIGONUCLEOTIDE SYNTHESIS

3,6,9-Trioxaundecane-1,11-diisocyanate (6) was synthesised from tetrae thylene glycol in 5 steps and 48 % overall yield. Spacer 6 was monofun ctionalised with the fully protected adenosyl-3'-O-succinate derivativ e 7 and linked to aminomethyl polystyrene (50 % crosslinked with divin ylbenzene) affording a solid support suitable for oligoribonucleotide synthesis (loading: similar to 20 mu mol/g). The HPLC analysis of a cr ude oligoribonucleotide synthesis and the isolated yield of purified o ligomer show that this spacer compares well to hexamethylene diamine. (C) 1998 Elsevier Science Ltd. All rights reserved.