TLC SEPARATION OF GLUCOPYRANOSYL SINAPATE AND OTHER PHENOLIC-COMPOUNDS FROM RAPESEED

Glucopyranosyl sinapate was obtained from rapeseeds using column chrom atography on Sephadex LH-20 and RP-18 semipreparative HPLC. TLC analys is of this compound, sinapic acid and phenolic compounds fractions fro m column chromatography were conducted on plates coated with silica ge l (standard and HPTLC), with cellulose and on RP-TLC plates. Eleven va rious solvent systems were applied to develop chromatograms. HPTL plat es were found more efficient than those coated with standard silica ge l. Compared to the latter cellulose ensured worse results of TLC analy sis. The best developing system for plates coated with silica gel was benzene-methanol-acetic acid (90:16:8). Separation of the compounds an alysed using RP-TLC depended on octadodecylsilanization degree of sili ca gel on TLC plate.