KINETIC DETERMINATION OF URIC-ACID IN URINE BASED ON SINGLE-LINE FLOW-SYSTEM WITH MULTISITE DETECTION

A flow injection system based on multi-site detection for kinetic spet rophotometric determination of uric acid in urine is described. The sy stem was studied regarding physical dispersion, elimination of sample matrix interferences and applicability to real samples. In the optimiz ed system, the sample is inserted into a water stream which merged dow nstream with the reagent solution containing iron(II) chloride and TPT Z. This system enables the determination of about 30 samples/h, and yi elds precise results (RSD usually <2.9%). Sensitivity is 212 mAU l/mmo l up to 0.60 mmol/l uric acid. Accuracy was assessed by running 32 sam ples already analyzed by a conventional enzymatic procedure. No statis tical difference between methods was found at the 95% confidence level .