LIPOSOME-DNA INTERACTION - MICROCALORIMETRIC STUDY

The authors applied differential scanning calorimetry (DSC) for studyi ng the thermodynamic characteristics of DNA-liposome interactions. At the first stage, the melting curves of the 'order-disorder' thermal tr ansition for lipid component and of the 'helix-coil' transition for DN A were obtained. At the second stage, the phase behavior of the DNA-li pid mixture as a function of both components (lipid/DNA ratio) was obt ained. The liposome-DNA interaction was investigated comparing the mel ting curves of the pure components and the mixture. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.