IMMUNOBLOTTING OF MULTIPLE ANTIGENIC PEPTIDES

Multiple antigenic peptides (MAPs) can be efficiently separated by sod ium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a nitrocellulose membrane for subsequent use in immunob lot (IgG and IgM). MAPs can be stained by Coomassie and silver on SDS- PAGE as well as by Fast Green on an immunoblot. Affinity immunoblottin g for analysis of antibody clonotype distribution has also been carrie d out using MAPs. High performance liquid chromatography purification of the MAPs is mainly responsible for their migration as sharp bands i n SDS-PAGE and immunoblotting.