RIBOSOMAL-PROTEIN L5 OF BOMBYX-MORI - CDNA SEQUENCE AND TISSUE DIFFERENCES IN THE L5 MESSENGER-RNA CONTENT

Authors
Citation
C. Yang et F. Sehnal, RIBOSOMAL-PROTEIN L5 OF BOMBYX-MORI - CDNA SEQUENCE AND TISSUE DIFFERENCES IN THE L5 MESSENGER-RNA CONTENT, Insect molecular biology, 7(4), 1998, pp. 301-306
Citations number
27
Categorie Soggetti
Biology,"Biothechnology & Applied Migrobiology",Entomology
Journal title
ISSN journal
09621075
Volume
7
Issue
4
Year of publication
1998
Pages
301 - 306
Database
ISI
SICI code
0962-1075(1998)7:4<301:RLOB-C>2.0.ZU;2-U
Abstract
A gene encoding the ribosomal protein L5, which assembles with the 5S rRNA into one of the components of the 60S ribosomal subunit, was iden tified in insects for the first time. We report on the isolation of Bo mbyx mori cDNA, whose putative translation product is 60-76% identical with the L5 sequences known from other animals and about 50% identica l with the L5 of rice and yeasts. Bombyx contains a single copy of the L5 gene, which is constitutively expressed as a 1.1 kb mRNA in all ti ssues. The ratio of L5 mRNA to total RNA appears to reflect the proteo synthetic tissue capacity. A very high level of L5 mRNA is maintained in functional silk glands, A rapid decline of the ratio of L5 mRNA to the rRNA, which occurs in the silk glands after cocoon spinning, indic ates differences in the stability of these two RNA classes.