CONFIRMATION THAT THIOBACILLUS-HALOPHILUS AND THIOBACILLUS-HYDROTHERMALIS ARE DISTINCT SPECIES WITHIN THE GAMMA-SUBCLASS OF THE PROTEOBACTERIA

Thiobacillus halophilus and Thiobacillus hydrothermalis share 98.7% si milarity in 16S rRNA sequence, possess similar gross DNA composition ( 64.2 and 67.4 mol% G+C values, respectively), and have similar physiol ogical properties. While this might have indicated that they were stra ins of a single species, DNA-DNA hybridization between the type strain s of the two species showed only 59% hybridization, indicating the org anisms to be different at the species level. Thiobacillus neapolitanus is the phylogenetically nearest neighbour of T. halophilus and T. hyd rothermalis (91.6-92.1% similarity in 16S rRNA sequence) and is the on ly other Thiobacillus in the gamma-subclass of the Proteobacteria that can be regarded as exclusively related to these two species. The 16S rRNA gene sequences of these three species are so different from those of the other thiobacilli in the gamma-subclass that they justify reco gnition as a distinct phyletic group. Their comparative properties are summarized.