VERSATILE EPITOPE TAGGING VECTOR FOR GENE-EXPRESSION IN MAMMALIAN-CELLS

Authors
Citation
T. Hosfield et Ql. Lu, VERSATILE EPITOPE TAGGING VECTOR FOR GENE-EXPRESSION IN MAMMALIAN-CELLS, BioTechniques, 25(2), 1998, pp. 306-309
Citations number
11
Categorie Soggetti
Biology,"Biochemical Research Methods
Journal title
ISSN journal
07366205
Volume
25
Issue
2
Year of publication
1998
Pages
306 - 309
Database
ISI
SICI code
0736-6205(1998)25:2<306:VETVFG>2.0.ZU;2-R
Abstract
We have constructed an epitope-tagging vector, pCMV-Tag I,for gene exp ression in mammalian cells. This vector which allows for N-terminal, C -terminal and internal ragging of the gene product of interest with th e FLAG(R) and/or c-myc epitopes, enables researchers to rapidly and ef ficiently characterize gene products in vivo.