MOLECULAR MAPPING OF THE CHROMOSOME-II BREAKPOINT OF T(11 17)(Q13 Q21) IN A T(11 14)(Q13 Q32)-POSITIVE-B NON-HODGKINS-LYMPHOMA

The FAU gene is the cellular homologue of the viral FOX sequences in t he genome of the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV); the viral FOX sequences have been shown to increase the transforming capacity of FBR-MuSV in vitro. The human FAU gene has recently been is olated, characterized, and mapped to chromosome band 11q13. Here, we r eport results of fluorescence in situ hybridization (FISH) analysis wh ich indicate that the FAU gene maps proximally to the putative oncogen e BCLI at 11q13. Furthermore, we identified a t(11;17)(q13;q21) transl ocation in tumor cells of a t(11;14)(q13;q32)-positive B-cell non-Hodg kin's lymphoma patient by FISH analysis using a FAU containing cosmid clone as molecular probe and by double-colour chromosome painting anal ysis using chromosome 11 - and chromosome 17-specific painting probes. The position of the chromosome 11 breakpoint of the t(11;17) transloc ation was pinpointed to a human DNA region around the FAU gene of abou t 40 kbp. (C) 1993 Wiley-Liss, Inc.