Fp. Han et al., VARIATION OF WHEATGRASS CHROMOSOMES IN WHEAT-WHEATGRASS ALIEN ADDITION LINE TAI-27 REVEALED BY FLUORESCENCE IN-SITU HYBRIDIZATION (FISH), SCIENCE IN CHINA SERIES C-LIFE SCIENCES, 41(4), 1998, pp. 367
The karyotype of the primary wheat-wheatgrass alien addition line TAI-
27 was 2n = 44 in which all of the chromosomes were metacentric and su
bmetacentric. However, in the progeny of TAI-27 a pair of chromosomes
had become small chromosomes in the two morphologically different plan
ts. Fluorescence in situ hybridization (FISH) technique was used to an
alyze the two different plants. The observations indicate that a pair
of small chromosomes in one variation line are from wheatgrass. In ano
ther variation line, a pair of small chromosomes are also from wheatgr
ass, while another pair of wheatgrass chromosomes have substituted the
wheat chromosomes. TAI-27 and its variant lines showed a high level o
f resistance to barley yellow dwarf virus (BYDV). The possible explana
tion for such a variation and the potential use of the variant lines w
ere discussed briefly.