DETERMINATION OF THE CONFORMATIONS OF CAMP RECEPTOR PROTEIN AND ITS T127L,S128A MUTANT WITH AND WITHOUT CAMP FROM SMALL-ANGLE NEUTRON-SCATTERING MEASUREMENTS

Small angle neutron scattering (SANS) measurements were performed on s olutions of cAMP receptor protein (CRP) and on solutions of the T127L, S128A double mutant of CRP (CRPQ) in D2O K3PO4 buffer containing 0.5 M KCI, in the absence and presence of 3',5' cyclic adenosine monophosp hate (cAMP). Energy-minimized structures of the CRP were calculated by minimization of the x-ray crystallographic structure of CRP in either the exclusively ''closed'' form where the alpha-helices of the carbox yl-terminal domain are folded close to the amino-terminal domain and i n the exclusively ''open'' form where the alpha-helices of the carboxy l-terminal domain are folded away from the amino-terminal domain. Neut ron scattering models show that the CRP SANS data follow closely the d ata curve predicted for unligated CRP in the open form, whereas the cA MP-ligated data are more in agreement with the data predicted for the minimized cAMP-ligated CRP structure in the closed form, Thus, it appe ars that CRP undergoes a conformational change from the open form to t he closed form in solution upon ligation with cAMP. The SANS data from the CRP and cAMP-ligated CRP*: are coincidental, which implies that there is very little structural difference between the two species of CRP. This is in agreement with in vivo results, which show that where as CRP activates transcription in the cell only in the presence of cAM P, CRP activates transcription in the absence of cAMP, implying that CRP is already in the correct conformation for the activation of tran scription.