C. Trocme et al., HUMAN B-LYMPHOCYTES SYNTHESIZE THE 92-KDA GELATINASE, MATRIX METALLOPROTEINASE-9, The Journal of biological chemistry, 273(32), 1998, pp. 20677-20684
Matrix metalloproteinases (MMPs) are involved in the remodeling of con
nective tissue as well as in disease states associated with acute and
chronic inflammation or tumoral metastatic processes. Despite detailed
and extensive studies of the mechanisms of lymphocyte extravasation,
remarkably little is known about the expression and regulation of meta
lloproteinases involved in the migratory process. By using zymography
and reverse transcription-polymerase chain reaction experiments, we ha
ve demonstrated that Epstein-Barr virus-immortalized B lymphocytes are
able to secrete a 92-kDa metalloproteinase with gelatinolytic activit
y which has been purified and identified as being MMP-9. Moreover, the
tissue inhibitor of metalloproteinase was shown to be constitutively
expressed by the B cells. The expression of 92-kDa gelatinase is media
ted by cytokines, growth factors, lipopolysaccharide, concanavalin A,
and the tumor promotor phorbol 12-myristate 13-acetate. Time dependenc
e activity increased rapidly up to 24 h of incubation with lipopolysac
charide or concanavalin A stimulation while it requires a delay and mo
re time to have an optimum effect when cytokines were the stimulating
agents; transforming growth factor-p abolished 92-kDa gelatinase produ
ction. Both staurosporine and wortmannin are inductive stimuli, and th
e level of MMP-9 secreted into the media is greater than that observed
with other agents except concanavalin A. Elicitation of the chemotact
ic migration of B cells through a model basement membrane by lipopolys
accharide was shown to be correlated with gelatinase expression and in
hibited by 7 mM captopril. Our study indicates that Epstein-Barr virus
-B lymphocytes express 92-kDa gelatinase, the production of which can
be modified by a variety of physiological and pharmacological signals
which have been shown to differ according to the cell type.