M. Vollebregt et al., ACTIVATION OF NF-KAPPA-B IN HUMAN NEUTROPHILS DURING PHAGOCYTOSIS OF BACTERIA INDEPENDENTLY OF OXIDANT GENERATION, FEBS letters, 432(1-2), 1998, pp. 40-44
We have exposed human neutrophils to opsonized Staphylococcus aureus a
nd used an electrophoretic mobility shift assay to show activation of
the transcription factor NK-kappa B above basal levels. Activation nas
evident within 10 min and was increased with higher bacteria:neutroph
il ratios. The neutrophil NADPH oxidase inhibitor diphenylene iodonium
, catalase, and other oxidant scavengers did not inhibit NF-kappa B ac
tivation, and no activation was seen with added hydrogen peroxide, Oxi
dants produced during phagocytosis, therefore, are not involved in the
activation mechanism, (C) 1998 Federation of European Biochemical Soc
ieties.