ACTIVATION OF NF-KAPPA-B IN HUMAN NEUTROPHILS DURING PHAGOCYTOSIS OF BACTERIA INDEPENDENTLY OF OXIDANT GENERATION

Citation
M. Vollebregt et al., ACTIVATION OF NF-KAPPA-B IN HUMAN NEUTROPHILS DURING PHAGOCYTOSIS OF BACTERIA INDEPENDENTLY OF OXIDANT GENERATION, FEBS letters, 432(1-2), 1998, pp. 40-44
Citations number
39
Categorie Soggetti
Biology,"Cell Biology",Biophysics
Journal title
ISSN journal
00145793
Volume
432
Issue
1-2
Year of publication
1998
Pages
40 - 44
Database
ISI
SICI code
0014-5793(1998)432:1-2<40:AONIHN>2.0.ZU;2-J
Abstract
We have exposed human neutrophils to opsonized Staphylococcus aureus a nd used an electrophoretic mobility shift assay to show activation of the transcription factor NK-kappa B above basal levels. Activation nas evident within 10 min and was increased with higher bacteria:neutroph il ratios. The neutrophil NADPH oxidase inhibitor diphenylene iodonium , catalase, and other oxidant scavengers did not inhibit NF-kappa B ac tivation, and no activation was seen with added hydrogen peroxide, Oxi dants produced during phagocytosis, therefore, are not involved in the activation mechanism, (C) 1998 Federation of European Biochemical Soc ieties.