During 1991 and 1992, 2,800 market-age pigs were sampled at federally
inspected abattoirs from across Canada. Anti-Toxoplasma gondii IgG at
titers of greater than or equal to 1:32 were found in 240 pigs examine
d by a commercial, latex agglutination test. Seroprevalences ranged fr
om 3.5 to 13.2% in the different regions of the country. Tissue hybrid
ization studies using a previously developed probe demonstrated T. gon
dii ribosomal RNA in 9 of 36 animals, whereas mouse bioassay testing o
f heart muscle and diaphragm from all 2,800 pigs failed to demonstrate
the presence of infective stages of T. gondii in tissues. Although se
rology results from this study indicated that Canadian market-age pigs
are infected with T. gondii at rates similar to those reported from o
ther parts of North America, mouse bioassay results suggested that Can
adian pork products contain low levels of infective organisms. This ap
parent discrepancy suggests that serological evidence of T. gondii inf
ection in pigs alone does not accurately assess the public health risk
s associated with consuming improperly cooked pork products.