GLUTATHIONE MEASUREMENT IN HUMAN PLASMA EVALUATION OF SAMPLE COLLECTION, STORAGE AND DERIVATIZATION CONDITIONS FOR ANALYSIS OF DANSYL DERIVATIVES BY HPLC
Dp. Jones et al., GLUTATHIONE MEASUREMENT IN HUMAN PLASMA EVALUATION OF SAMPLE COLLECTION, STORAGE AND DERIVATIZATION CONDITIONS FOR ANALYSIS OF DANSYL DERIVATIVES BY HPLC, Clinica chimica acta, 275(2), 1998, pp. 175-184
Literature values for human plasma GSH vary over 10-fold despite the u
se of apparently valid analytical procedures for GSH measurement. The
purpose of this study was to develop a procedure to minimize error in
sample collection, processing and storage that could contribute to suc
h differences. HPLC with fluorescence detection of dansyl derivatives
was used for quantification. The results show that collection of blood
with a butterfly needle and syringe reduces overestimation due to lim
ited hemolysis and that use of a preservation solution designed to inh
ibit autooxidation and enzymatic degradation allows quantitative recov
ery of both GSH and GSSG. Stability tests showed that non-derivatized
samples were stable for at least 2 months at - 80 degrees while dansyl
derivatives were stable in the dark at 0-4 degrees for 12 months. Res
ults from 59 healthy individuals (20-43 years) provided a mean (+/-1 S
D) GSH value of 2.09 +/- 1.14 micromolar. (C) 1998 Elsevier Science B.
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