Southern hybridisation with a single microsatellite probe, (TCT)(10),
sufficed to discriminate between a representative set of cultivars/acc
essions of lettuce, Lactuca sativa L., and its wild relatives L. serri
ola, L. saligna and L. virosa. Variability within cultivars was tested
in a relatively modern cultivar (Hector), where no variation was foun
d, and in an older and morphologically more variable cultivar (Madrile
ne), where heterogeneity was observed in the TCT fingerprint. (TCT)(10
) fingerprinting should be useful for variety identification and homog
eneity testing in lettuce.