In this short review, recent investigations of bovine in vitro fertili
zation are discussed. The technical and biological influences to maxim
ise the oocyte yield are of particular importance especially for the c
ollection of oocytes. Embryo quality is affected by the culture condit
ions,, in that the smaller number of inner cell mass cells reduces the
ir quality. Also, serum and cell containing media are possible sources
of viral infections for embryos. For example, bovine herpesvirus 1 an
d bovine viral diarrhoea virus can readily infect and replicate in emb
ryonic cells. Further research arch is required on the development of
sl specific media to prevent viral infection? and to improve embryo qu
ality.