IC101, EXTRACELLULAR-MATRIX ANTAGONIST PRODUCED BY STREPTOMYCES SP MJ202-72F3 - PRODUCTION, ISOLATION, STRUCTURE DETERMINATION AND BIOLOGICAL-ACTIVITY

In our search for inhibitors of cell adhesion to components of extrace llular matrix (ECM), fibronectin, laminin and collagen type IV, we suc ceeded in finding a novel cyclic hexadepsipeptide antibiotic, named IC 101, which was isolated from cultured mycelium of Streptomyces albulus MJ202-72F3. It was purified by centrifugal partition chromatography, preparative reverse phase HPLC and Sephadex LH-20 and was obtained as a white powder. IC101 strongly inhibited cell adhesion to ECM componen ts, suppressed immune responses in vitro and in vivo, and exhibited an timicrobial activity on Gram-positive bacteria.